Do you've got a protocol or instructed antibodies to carry out ICC staining on my completely differentiated ALI cultures?
Blend gently before transferring cells to the conical tube made up of 10 mL of pre-warmed wash medium. Take note: Clean the pipette tip and cryovial Along with the clean medium at the least 3 times to aid improve recovery.
New to STEMCELL? Sign up for an account to immediately and simply purchase solutions on the internet and for 1-simply click use of all educational content material. Build an Account
Get the job done rapidly at this action to pool wells right into a 15 mL tube. Be aware: For a considerable-scale dissociation, make use of a multichannel pipette to pool cells into a sterile reagent reservoir. Include the pooled wells right into a fifteen mL tube.
New to STEMCELL? Register for an account to speedily and simply buy solutions online and for one particular-simply click entry to all academic content.
Make a lot more informed paying for selections with our new item availability and supply estimate attribute, now accessible on all products webpages, inside your cart, and during checkout.
Likely again towards the plate, rinse Every single very well with one mL of FACS buffer and transfer the volume for the 15 줄기세포 지방이식 mL tube. Note: Preserve cell suspension on ice immediately after transfer to the tube right up until 자가지방이식 wanting to operate FACS.
I agree to acquire potential Digital communications from STEMCELL. I accept which i can unsubscribe stem cell clinic any time.
To modify your shopping cart, make sure you take out the current quote purchase before making alterations 지방흡입 to the cart. When you have to have improvements to your estimate, you should Speak to your local product sales representative. Cancel
New to STEMCELL? Sign-up for an account to immediately and simply acquire items online and for a person-click on access to all instructional information. Generate an Account
When planning guidebook RNAs, it is recommended to choose sequences that align Together with the transcription get started web-site on the gene of interest in which achievable.
Complete cell counts working with an automatic cell counter. Note: Reduced viability is often optimized through the size of collagenase or ACCUTASE™ incubation. It can even be optimized by reducing the volume of triturations.
Thank you on your curiosity On this solution. 줄기세포 지방이식 Be sure to deliver us with all your Call facts and your neighborhood consultant will Get in touch with you having a customized quotation. Wherever acceptable, they also can help you that has a(n): Believed supply time on your location Product sample or unique provide In-lab demonstration By submitting this form, that you are supplying your consent to STEMCELL Technologies copyright Inc.
To switch your procuring cart, be sure to clear away The existing estimate purchase before making changes to your cart. If you involve improvements to your estimate, make sure you contact your neighborhood profits consultant. Cancel